Chronic effects such as reproduction and population dynamics with elevated CO2 concentration were evaluated using two representative marine benthic species, copepod (Tisbe sp.) and amphipod (Monocorophium acherusicum) adopting long-term exposure. Juvenile copepod and amphipod individuals were cultivated in the seawater equilibrated with control air (0.395 mmol CO2/air mol) and high CO2 air having 0.998, to 3.03, 10.3, and 30.1 mmol CO2/air mol during 20 and 46 days, respectively. After the exposure period, the number of benthic invertebrate was counted with separate larval and juvenile stage such as naupliar, copepodid and adult for copepod, or neonate and adult for amphipod, respectively. The individual number of both test species at each life-stage was significantly decreased in seawater with 10.3 mmol CO2/air mol or higher. Recently, the technology of marine CO2 sequestration has been developed for the reduction of CO2 emission, which may cause climate change. However, under various scenarios of CO2 leaks during the injection process or sequestrated CO2 in marine geological structure, the potential risk to organism including various invertebrates can be expected to exposure. So the results of this study suggested that the detailed consideration on the adverse effect with marine ecosystem can be prerequisite for the marine CO2 sequestration projects.

Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 실험재료
  2.2. 실험방법
  2.3. 자료의 분석
 3. 결 과
  3.1. 해수의 pH
  3.2. 저서성 요각류에 대한 영향
  3.3. 저서성 단각류에 대한 영향
 4. 고 찰
 5. 결 론
 참 고 문 헌

• 문성대((주)네오엔비즈 환경안전연구소, 충남대학교 생명시스템과학대학 생물과학과) | Seong-Dae Moon (Institute of Environmental Protection and Safety, NeoEnBiz Co., Department of Biological Science, School of Biological Sciences and Biotechnology, Chungnam National University)
• 최태섭((주)네오엔비즈 환경안전연구소) | Tae Seob Choi (Institute of Environmental Protection and Safety, NeoEnBiz Co.) Corresponding author
• 성찬경((주)네오엔비즈 환경안전연구소, 서울시립대학교 에너지환경시스템공학과) | Chan-Gyoung Sung (Institute of Environmental Protection and Safety, NeoEnBiz Co., Department of Energy and Environmental System Engineering, University of Seoul)
• 이정석((주)네오엔비즈 환경안전연구소) | Jung-Suk Lee (Institute of Environmental Protection and Safety, NeoEnBiz Co.)
• 박영규(한국해양과학기술원 해양순환기후연구부) | Young-Gyu Park (Ocean Circulation and Climate Research Division, Korea Institute of Ocean Science and Technology(KIOST))
• 강성길(한국해양과학기술원 해양시스템안전연구소) | Seong-Gil Kang (Maritime and Ocean Engineering Research Institute(MOERI)/Korea Institute of Ocean Science and Technology(KIOST))