To evaluate the performance of a new automated coliform enumeration system (TEMPO® CC) for the quantitative test of coliform bacteria contaminated in domestic livestock processed foods, a total of 507 samples of livestock foods were tested by the TEMPO® CC method, the most probable number (MPN) method, and Petrifilm method, respectively. The results of those three methods were compared to each other. Of 507 samples of livestock processed foods used in this study, 217 samples were contaminated artificially with coliform bacteria and the rest (n=290) were contaminated naturally. The results of the TEMPO® CC method for all samples were equivalent to those obtained from the MPN method, except 8 samples. In addition, 496 (97.8%) out of 507 samples made agreement between the TEMPO® CC method and the Petrifilm method. The correlation coefficients between TEMPO® CC and MPN methods as well as between TEMPO® CC method and Petrifilm method were above 0.9, and the slope and intercept of the linear regression model was different in less than 1 value. In conclusion, there were statistically equivalent levels of performance between the TEMPO® CC and the reference and alternative methods for the enumeration of coliform bacteria in livestock processed foods in this study.

ABSTRACT
 Introduction
 Materials and Methods
  1. Sample preparation
  2. Artificial coliform bacteria inoculation to livestock processedfoods
  3. Enumeration of coliform bacteria using the MPN, thePetrifilm and the TEMPO® methods
  4. Inter-laboratory test
  5. Data analysis
 Results
  1. Degree of agreement between the MPN method andthe TEMPO® CC method
  2. Degree of agreement between the Petrifilm methodand the TEMPO® CC method
  3. Inter-laboratory analysis for the correlation coefficientbetween the quantitative standard test methods and theTEMPO® CC method
 Discussion
 References

• 김영조(국립수의과학검역원) | Young Jo Kim (National Veterinary Research and Quarantine Service)
• 위성환(국립수의과학검역원) | Sung Hwan Wee (National Veterinary Research and Quarantine Service)
• 윤하정(국립수의과학검역원) | Ha Jung Yoon (National Veterinary Research and Quarantine Service)
• 허은정(국립수의과학검역원) | Eun Jeong Heo (National Veterinary Research and Quarantine Service)
• 박현정(국립수의과학검역원) | Hyun Jung Park (National Veterinary Research and Quarantine Service)
• 김지호(국립수의과학검역원) | Ji Ho Kim (National Veterinary Research and Quarantine Service)
• 문진산(국립수의과학검역원) | Jin San Moon (National Veterinary Research and Quarantine Service) Correspondence