Random amplified polymorphic DNA (RAPD) and fluorescent amplified fragment length polymorphism (FAFLP) analyses were executed on a total of 28 Salmonella spp., including 6 ATCC reference strains, 2 isolates from outbreaks of food poisoning in Gwangju, and 20 isolates from carcasses. For RAPD analysis, four primers, named P1254, 23L, OPA-4, OPB-17 were used producing amplification fragments ranged from 0.18kb to 2.6kb. As a result, 5 types using P1254, 5 types using 23L, 3 types using OPA-4, and 6 types using OPB-17 and a total of 18 RAPD types were achieved. For FAFLP analysis, bacterial genomic DNA was digested with endonucleases EcoRⅠ and MseⅠ, site-specific adaptors were ligated, and PCR amplification was carried out with an EcoR1 adaptor-specific primer labelled with fluorescent dye. Amplified fragments, which were separated on a polyacrylamide sequencing gel ranged from 35bp to 300bp were analysed. Results were displayed as a dendrogram with genetic distance. Twenty two Salmonella isolates and 6 reference strains were divided into 14 groups in a level of 0.136 genetic distance. In conclusion, Salmonella isolates of chicken carcasses have different genetic properties when compared to reference strains and isolates from outbreak of food poisoning.

ABSTRACT
 서 론
 재료 및 방법
  1. 시험 균주
  2. 균 배양 및 Genomic DNA 추출
  3. RAPD를 위한 Primers, PCR 및 분석
  4. FAFLP를 위한 Enzyme restriction과 ligation
  5. FAFLP를 위한 Pre-selective와 Selective PCR
  6. 310 Genetic Analyzer를 이용하여 FAFLP의 DNA 단편의 분석
 결 과
  1. RAPD
  2. FAFLP
 고 찰
 참고문헌

• 박신정(전라남도 생물의약연구센터) | Sin Jeong Park (Jeonnam Biopharmaceutical Research Center)
• 이재일(전남대학교 수의학과대학) | Jae Il Lee (College of Veterinary Medicine, Chonnam National University) Correspondence