Cryopreservation is mainly used for preservation of boar sperm. However, this method stresses the sperm by reactive oxygen species (ROS), and the conception rate and the litter size are not more efficient than the liquid preservation of spermatozoa. Therefore, we use chitosan which is a natural product derived antioxidant compound. We used GnHA and GnHG as chitosan complexes to cryopreserve boar sperm for improve sperm metabolism and function.
Sperm parameter (sperm motility, progressive motility, path velocity, straight-line velocity, curvilinear velocity) is measured by computer-assisted sperm analysis (CASA) using frozen sperm with GnHA or GnHG (0, 0.25, 0.5, 1 mg/mL). Also, lipid peroxidation analysis using malondialdehyde (MDA) is performed to confirm the antioxidative effect of chitosan in frozen spermatozoa.
Sperm motility was higher in GnHA 0.25 mg/mL and GnHG 0.5 mg/mL compared to control. In addition, GnHG 0.5 mg/mL was significantly decreased in lipid peroxidation analysis.
The results suggest that GnHA and GnHG are effective for boar sperm cryopreservation by antioxidant effect.

• Hye-Min Hong(Department of Life Science, College of Bio-nano technology, Gachon University)
• Ga-Young Sim(Department of Life Science, College of Bio-nano technology, Gachon University)
• So-Mi Park(Department of Life Science, College of Bio-nano technology, Gachon University)
• Eun-Joo Lee(Department of Life Science, College of Bio-nano technology, Gachon University)
• Dae-Young Kim(Department of Life Science, College of Bio-nano technology, Gachon University)