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Expression of Cdx2, Oct4, Sox2, Nanog and E-cadherin in porcine parthenogenetic and SCNT embryos

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한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

The embryonic genome activation (EGA) is genetically activated states that embryos make the materials such as growth factors for using themselves. EGA is various because they have many materials, different site, different stage, also different species. At this time, transcription factors are expressed. Transcription factors bind to specific DNA region, and regulate the gene expression. Thus, we check the expression of transcription factors, we can know that embryo development is very well or not. The development stages of embryos are basically the stages from fertilization to blastocyst. So, we check the embryos oocyte to blastocyst. In our experiments, we focus the early developmental transcription factors such as Cdx2, Oct4, Sox2, Nanog and E-Cadherin. Above antibody factors showed different expression sites, and there were many differentiated parts from other animal species. In addition, we compared the SCNT and parthenogenetic activation (PA) because these are same methods using electrical activation among the embryo production methods. Our results showed not only similar patterns but also different patterns between pig and mouse. Therefore, we have to investigate that different patterns of transcription factors play a role in pigs, and why occur.

저자
  • Joo Bin Lee(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)
  • Tao Lin(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)
  • Reza K. Oqani(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)
  • Hyeon Yeong Shin(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)
  • Jae Eun Lee(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)
  • Jung won Kang(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)
  • So Yeon Kim(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)
  • Dong Il Jin(Department of Animal Science & Biotechnology, Chungnam National University, Daejeon, Republic of Korea)