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배초향 꽃 단계 별 페닐프로파노이드 HPLC 분석 및 생합성 유전자 발현 분석

Expression Levels of Phenylpropanoid Biosynthesis Genes and Phenylpropanoid Accumulation in Agastache rugosa Flowers at Different Developmental Stages

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  • URLhttps://db.koreascholar.com/Article/Detail/362826
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한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background : Agastache rugosa (A. rugosa), belonging to the Lamiaceae family, is a medicinal plant mainly distributed in Korea and contains various phenolic compounds revealing anti-fungal and anti-HIV properties. This study is aim to investigate change in phenylpropanoid content of flowers at different developmental stages using high performance liquid chromatography (HPLC) and quantitative real time polymerase chain reaction (qRT-PCR).
Methods and Results : The variation in the transcriptional level of phenylpropanoid biosynthetic genes and phenylpropanoid contents in the flowers of A. rugosa at different developmental stages was analyzed. The transcript levels of phenylpropanoid biosynthesis genes, including ArPAL (phenylalanine ammonia-lyase), ArC4H (cinnamate 4-hydroxylase), and ArCHS (Chalcone synthase), were high in flowers at 1st stage compared with flowers at 2nd and 3rd stages. On the other hand, the expression levels of flavonoid biosynthesis genes, including ArTAT (tyrosine amino transferase), ArHPPR (hydroxyl phenylpyruvate reductase), and ArRAS (rosmarinic acid synthase), were higher in flowers at 3rd stage than those of flowers at 1st and 2nd. These results were consistent with HPLC analysis revealing that most phenolic compounds were higher in flowers at 1st and 2nd stage but the level of rosmarinic acid was the highest in 3rd stage.
Conclusion : Our findings provide the information on change in phenylpropanoid biosynthesis in A. rugosa flowers at different developmental stages.

저자
  • 박창하(충남대학교 식물자원학과) | Chang Ha Park
  • 박예은(충남대학교 식물자원학과) | Ye Eun Park
  • 박상언(충남대학교 식물자원학과) | Sang Un Park Corresponding author
  • 최가희(영농조합법인 부일식품) | Ga Hee Choi