Background : Gastrodia elata Blume (G. elata) is important medicinal resource in korea. Gastrodin and 4-hydroxybenzyl alcohol (4-HBA) are major active compounds of G. elata, and ρ-cresol is major cause of off-odor like pig slurry from G. elata. The off-odor can decrease the quality of fresh G. elata as well as its products. Therefore, this study was performed to investigate the influence of extraction temperature on bio-active and odorous compounds of G. elata extract.
Methods and Results : G. elata was extracted with distilled water at 0, 30, 60, and 90℃ for 20, 40, 60, and 120 min. Gastrodin and 4-HBA contents were analyzed by using a HPLC-UVD, and ρ-cresol content was analyzed by using a SPME-GC-MS. Gastrodin content increased as increasing extraction temperature and time, and showed the highest value in extract at 90℃. 4-HBA content showed the highest value at 60℃, and increased as increasing extraction time. Total content of gastrodin and 4-HBA was higher in extract from G. elata at 60℃ for 120 min than other extracts. ρ-Cresol content was varied according to extraction temperature, and was lower in extract at 30 and 60℃ than 0 and 90℃.
Conclusion : These results indicated that the extraction temperature can affect the bio-active components and off-odor of G. elata extract, and 60℃ is appropriate to improve the qualities including bio-active component and off-odor of G. elata extract and its products.