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Background : Aralia continentalis Kitag. (syn. = A. cordata Thunb., Araliaceae) is a traditional medicinal herb spread widely in northeastern Asia, including Korea, China, and Japan. Many constituents from its root extracts, including diterpene and essential oils, have been isolated as active components for antioxidant, anti-inflammatory, analgesic, sedative, antifungal, anti-thrombotic, and growth inhibition. In this study, we describe the structural determination of the two new compounds.
Materials and Results : The roots of A. continentalis were collected in Namwon-si, Jeollabuk-do, Korea, in March 2018 and identified by one of the authors (Dr. S. S. Hong). The roots of A. continentalis were extracted with 70% EtOH two times at room temperature. The concentrated residue was subsequently suspended in H2O and partitioned with n-hexane, CH2Cl2, EtOAc and n-BuOH. The n-hexane, CH2Cl2, and EtOAc layer were subjected to sequential column chromatography over silica-gel, RP-18, MPLC and preparative HPLC to isolated the compounds 1 - 30. Consequently, a new nor-ent-pimaran diterpenoid (11) and a new 8-O-4′ type neolignan (25) along with 28 known compounds that included diterpenoids, phenolic derivates, and polyacetylenes have been isolated from the ethanol extract of the roots of A. continentalis. The structures of the new compounds were established by spectroscopic data interpretation, particularly HRESIMS, 1D and 2D NMR data including HSQC and HMBC. Also, those of the known compounds were identified by spectral comparison with those of the reported values.
Conclusion : The structures of two new compounds were determined as 18-nor-ent-pimara-9 (11),15-diene-4β-ol (11) and 8-O-4-dehydrocoumaroyl-ferulic acid (25). To our knowledge, compounds 10, 14, 15, 19, 20, 22 – 24, and 26 were isolated and identified from Aralia genus for the first time.
