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Background : Ligusticum chuanxiong Hort is a perennial herb of the Umbelliferae family and an important traditional oriental medicinal plant. The compounds contained in L. chuanxiong can be divided into five kinds, essential oil, alkaloids, phenolic acids, phthalide lactones, and other constituents. These compounds have cardiovascular and cerebrovascular effects, antioxidants, neuroprotection, anti-fibrinolytic, antidotes, anti-inflammatory and antitumor activities. In this study, we anticipated to establish the in vitro propagation system of L. chuanxiong, which is a high economic value as medicinal herb, by plant tissue culture to solve the problem of root stocks contamination.
Methods and Results : The whole study was carried out in the department of Herbal crop research, Eumseong, RDA. In this study, L. chuanxiong nodes was used as an explant and it was surface sterilized by 2% sodium hypochlorite for 1 minutes, then washed with ddH2O several times. Further the surface sterilized nodes were placed on MS basal media. Multiple shoots were induced on MS, SH, WPM media with 0.1 - 2 ㎎/ℓ auxin (NAA, IBA) and cytokine (BA). In this study we obtained 4.6 multi-shoots per an explant, and growth of the shoot was also favorable in the presence of 1.0 ㎎/ℓ BA. Subsequent transfer of these regenerated shoots on 1/2 MS media resulted in root formation. The rooted plantlets were able to grow in soil after 3 weeks of acclimatization.
Conclusion : The optimal conditions for in vitro propagation of L. chuanxiong were established through this experiment.
