논문 상세보기
pp.661-676
Veronica persica (V. persica) is a perennial plant that is broadly distributed in Europe, Asia and so on. V. persica is used for pain about the lower abdomen and low back. The aim of this study was to investigate the anti-oxidant and antiinflammatory effects of V. persica ethanol extract in LPS-induced RAW 264.7 cells. To evaluate the anti-oxidant activity, the DPPH and ABTS radical scavenging, total polyphenol and flavonoid contents, and reducing power activity were carried out. The DPPH and ABTS radical scavenging activity were evaluated as 72.0% and 73.0% at the concentrations of 200 and 500 μg/mL, respectively. Total polyphenol and flavonoid contents of V. persica extracts were measured as 65.22 mg/g and 43.82 mg/g at the concentration of 1 mg/mL. The reducing power activity measurement showed 53.0% activity at 1 mg/mL. The anti-inflammatory effects of the V. persica extract were evaluated in LPS induced RAW 264.7 cells. In the evaluation of cell viability by proliferation ＆ cytotoxicity assay kit, the cytotoxicity of the extract was not confirmed at 0~800 μg/mL concentration. And the V. persica significantly inhibited NO production in a concentration dependent manner. The inhibition effects of NO in cell medium of V. persica was over 80% at 800 μg/mL. The V. persica also suppressed the expression of iNOS, COX-2, and phosphorylation of NF-κB and IkB-α proteins. These results indicate that the V. persica has anti-oxidant and anti-inflammatory effects by modulating NF-κB signaling pathways and can be used as natural functional materials.
Ⅱ. 재료 및 방법
1. 시료 채집
2. 추출용 시료
3. 총 폴리페놀 함량 측정
4. 총 플라보노이드 함량 측정
5. DPPH radical 소거능 측정
6. ABTS radical 소거능 측정
7. 환원력 측정
8. 세포배양
9. 세포 생존율
10. NO 생성량
11. Western blotting
12. 통계처리
Ⅲ. 결과 및 고찰
1. 총 폴리페놀 및 플라보노이드 함량
2. DPPH 및 ABTS radical 소거능
3. 환원력
4. 세포 생존율
5. NO 생성량
6. iNOS 및 COX-2 단백질 발현량
7. p-NF-κB p65 및 p-IkB-α 단백질 발현량
Ⅵ. 적 요
References
