The objective of this research was to investigate the removal efficiencies of VOCs and odors with newly developed biofilter which was designed to sustain the biofilm constantly on the packed media. Initially, four types of media, for example, fiber, activated carbon, ceramic and the mixture of activated carbon and ceramic(A/C mixture), were used for packed materials of biofilter. When ethylalcohol was selected as a test gas for media efficiency, fiber and A/C mixture had better removal efficiencies of ethylalcohol than others. Removal efficiencies for acetaldehyde, ethylalcohol, butylalcohol, ethylacetate and diethylamine in biofilter with fiber and A/C mixture as packed media were increased as the residence time increased. Butylalcohol, especially, showed the maximum removal efficiency among all used VOCs and odors. In case of ethylacetate, the difference of removal efficiencies between low and high residence times was wide remarkably.