To find best humidity condition for the use of Beauveria ARP14 against Grapholita molesta (Busck), first instar of G. molesta was treated with three conidial concentrations (1×107, 1×108, and 1×109 conidia/ml) in three different relative humidities (RH; 55, 75, and 95%). Mortality varied depending on concentration and humidity. Mortality in 95% RH was 1.5 and 2.7 times higher than 75 and 52% humidity at 1×109 conidia/ml and 16 hours after the exposure. However, the mycosis rates after 14 days were all not different among the treatments. In conclusion B. bassiana ARP14 could be most effective when applied higher at RH condition.