Trialeurodes vaporariorum is an important greenhouse pest worldwide. We compared the efficacy of a new strain of Beauveria bassiana (ARP14) with a commercialized one (GHA) against 2nd instar and 4th instar of T. vaporarioum. Those nymphs were exposed to the fungus in a concentration of 1×108 conidia/mL by leaf dipping method. There was no difference in mycosis rates at any observation time between the two strains in both 2nd (100.0%) and 4th instar (100.0%). Lethal median time (LT50) wasn't different between the two strains in both 2nd and 4th instar. In conclusion, the new B. bassiana strain (ARP14) would be a good candidate of mycoinsecticide for whitefly management.

Bemisia tabaci (Gennadius) is an important polyphagous pest and transmits plant pathogenic virus to numerous agriculture crops. We compared the efficacy of Beauveria bassiana ARP14 strain with commercialized GHA strain against 2nd instar and 4th instar of B. tabaci. The nymphs were exposed in 1×108 conidia/mL by dipping method. There was no difference in mycosis rate between the two strains in both 2nd and 4th instar. After 72 h, mycosis rate of 2nd instar was 100.0% in B. bassiana ARP14 strain followed by 97.4% in B. bassiana GHA strain. In 4th instar, B. bassiana ARP14 strain caused 100.0% while B. bassiana GHA strain caused 93.3%.

The hawthorn spider mite, Amphitetranychus viennensis (Zacher), is an important tetranychid causing damage on fruittrees and woody ornamental plants. We investigated the effect of a mycoinsecticide BotaniGard® ES at different humiditylevels on the eggs and adults mortality of A. viennensis. In a laboratory condition, egg mortality was significantly influencedby the conidial concentration and RHs. Highest mortality of eggs was observed at 1×108 conidia/ml with 95% RH thanother conditions. In 1×108 conidia/ml, LT50 at 95 and 75% RH were significantly different from 55% RH. In BotaniGard®spray in cherry plants, mites decreased on day 6 at all the concentrations, but the mite populations rebounded exceptin 1×109 conidia/ml where it maintained 0 until 9 days after spraying. It is therefore concluded that B. bassiana hasa potential to be used against A. viennensis

A mycoinsecticide, Beauveria bassiana (strain GHA) marketed as BotaniGard® ES, was evaluated as a biological control agent against Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) a major soybean pest. It was tested on commercial soybean fields with one time spray of both high (1×109) and medium (1×108spore/ml) concentration and double spray of the medium concentration. Nymph population decreased in all treated fields regardless of dose and frequency of spray. But, adult and egg populations were not affected. Alive nymphs collected from the fields and kept in laboratory showed infection rate ranging 35-68% in treated plots while 0% in control plots. It also reduced damage on soybean pods and seeds by 17.7-21.2 and 18.0-22.5% compare to control, respectively. Additionally, B. bassiana reduced other pests found in soybean fields including brown marmorated stink bug Halyomorpha halys (Stål) and two striped leaf beetle Medythia nigrobilineata Motschulsky. Also there was no negative effect on the parasitism of released non-viable eggs of R. pedestris. These results suggest that, in field condition, one time spraying of B. bassiana in the concentration of 1×108spore/ml can help reducing crop damage as well as R. pedestris nymph population.

Lethal concentration of Beauveria bassiana (Strain GHA, Botanigard, Arysta LifeScience) against Riptortus pedestris (F.) (Hemiptera: Alydidae) was compared with that of its’ parasitoid Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae). Five concentrations of the mycoinsecticide, i.e., 5×108, 1×108, 2×107, 4×106, and 8×105 conidia/ml, were used. Glass scintillation vial was coated with 100 μl solution, and insects were released and assessed up to 12 hours. The LC50 and LC20 of second instar R. pedestris were 1.8×1010 and 1.5×108 conidia/ml, and those of O. nezarae were 1.9×107 and 2.8×106 conidia/ml, respectively. The mortality of O. nezarae was observed 3.9 times higher than R. pedestris in higher concentration. This result shows that O. nezarae is more susceptible to the mycoinsecticide than R. pedestris.