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First report of Bursaphelenchus sinensis on Pinus densiflora in Korea and pathogenicity test
Bursaphelenchus sinensis was first found in Austria from the wood packaging material imported from China. In Korea, B. sinensis was detected from dead branches of Pinus densiflora located in Jinju, Gyeongnam province. Morphology of B. sinensis was characterized by unique male spicule with weak rostrum and squared condylus, and female with small vulval flap and conical shaped tail. Genomic DNA of B. sinensis was extracted and ITS region was fully amplified by PCR. ITS-PCR product was analyzed by RFLP and also directly sequenced. Polymorphism by RFLP-ITS was matched with that of B. sinensis and ITS sequence data was identical to B. sinensis information in GenBank. The Korean isolate of B. sinensis, Bs-Jinju, was submitted to GenBank and acquired the accession number, #MG934676. Host pathogenicity of B. sinensis was tested by artificial inoculation of nematode (30,000/ sapling) on 5-year-old P. densiflora. There were no disease symptoms in all tested pines inoculated by B. sinensis, whereas 100% mortality showed on pine saplings by B. xylophilus inoculation. This is the first report of B. sinensis in South Korea.
