Here, we provided a lucid key for twelve lepidopteran quarantine pests in South Korea. They were selected based on border quarantine inspection data from 1996 to 2016 in S. Korea. They are belonging to eight families: Carposinidae, Crambidae, Pyralidae, Gelechiidae, Oecophoridae, Tineidae, Tortricidae and Geometridae, and also categorized into the three groups: prohibited, regulated and nonquarantine pests. The key includes diagnostic characters from head, thorax, fore- and hind-wings for each species examined.