The purpose of this study was to investigate the effect of somatotype on the VO2max and hormone (adrenaline and noradrenaline) during treadmill walking. Forty healthy men participated and were randomized to four groups: Male 1 (M1) group, Male 2 (M2) group, Male 3 (M3) group, and Male 4 (M4) group. M4 group is the largest body type, and M1 group is the smaller the body type. Participants walked at a speed of 3.5 km/h for five minutes at an incline angle of 0°, 5°, and 10° in the treadmill. Maximum oxygen consumption and hormone (adrenaline and noradrenaline) were measured. In the results, VO2max has significantly increased according to the degree of the treadmill inclination, and M4 group (larger body type) consumed more oxygen than the M1 group (smaller body type). In the hormone, there was a significant increase in adrenaline concentration after walking in all groups, and there was a significant difference in M1-M4, M2-M4 and M3-M4. The noradrenaline concentration significantly increased after treadmill gait in all groups, and there was no significant difference in noradrenaline between groups. This study suggests that the larger body type consumes more oxygen during walking, and treadmill walking contributes to an increase in the concentration of adrenaline and noradrenaline.