In this study, bacterial growth was assessed by flow cytometry analysis of fluorescent probes-stained bacteria. Flow cytometry has many advantages of rapid analytical time, a low standard deviation, and highly sensitive detection of live and Dead E.coli over colony forming assay. When untreated bacteria were stained by using Thiazole Orange (TO) and Propidium Iodide (PI), double staining had a short analytical time as compared with that of single staining while its error rate was similar to that of single staining. Through double staining experiments, it was determined that optimal concentrations for TO and PI staining were 420 nM and 9.6 μM, respectively.

Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 대장균 배양
  2.2. 평판계수방법과 유세포 분석기를 이용한 대장균계수
  2.3. 유세포 분석기를 사용한 대장균 분석방법
 3. 결과 및 고찰
  3.1. 평판계수방법과 유세포 분석기를 이용한 대장균계수
  3.2. 유세포 분석기의 분석조건 설정
 4. 결 론
 REFERENCES

• 김지혜(계명대학교 환경과학과) | Ji-Hye Kim (Department of Environmental Science Keimyung University)
• 박상원(계명대학교 녹색융합기술연구소) | Sang-Won Park (Center for Green & Fusion Technologies, Keimyung University) Corresponding author
• 조영식(계명대학교 약학대학 약학과) | Young Sik Cho (College of Pharmacy, Keimyung University)