This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

• Lauren Togonon Arayan(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Tran Xuan Ngoc Huy(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Alisha Wehdnesday Bernardo Reyes(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Huynh Tan Hop(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Huynh Tan Hop(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Vu Hai Son(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• WonGi Min(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Hu Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author