Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine to treat inflammatory diseases in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprotein) in human intestinal epithelial INT-407 cells. We demonstrated that UDN glycoprotein (20 μg/mL) could inhibit the production of lactate dehydrogenase (LDH) induced by toluene, an ecotoxic substance. Additionally, we found that the toluene-induced intestinal cytotoxicity was mediated by the phosphorylation of p38 Mitogen-Activated Protein Kinase (MAPK) via the production of intracellular Reactive Oxygen Species (ROS). The UDN glycoprotein significantly decreased the levels of ROS production and p38 MAPK activation in toluene-stimulated INT-407 cells. Moreover, the UDN glycoprotein inhibits the phosphorylation of nuclear factor-kappa B (NF-κB), which is responsible for the production of LDH, in toluene-stimulated INT-407 cells. Collectively, our data indicate that UDN glycoprotein is a natural antioxidant and a modulator of ecotoxicity signaling pathways in human intestinal epithelial cells.

Abstract
 1. 서 론
 2. 재료 및 실험방법
 3. 결과 및 고찰
  3.1. 느릅나무 당단백질의 환경독성 제어효과
  3.2. 활성산소종 생성 억제를 통한 느릅나무 당단백질의환경독성 제어효과
  3.3. p38 MAPK의 인산화 억제를 통한 느릅나무당단백질의 환경독성 제어효과
  3.4. NF-κB의 인산화 억제를 통한 느릅나무 당단백질의환경독성 제어효과
  3.5. 느릅나무 당단백질의 환경독성 세포신호전달 기전제어효과
 4. 결 론
 REFERENCE

• 김도완(대구한의대학교 제약공학과) | Do-Wan Kim (Department of Pharmaceutical Engineering, Daegu Haany University)
• 김지윤(대구한의대학교 제약공학과) | Ji-Yun Kim (Department of Pharmaceutical Engineering, Daegu Haany University)
• 박문기(대구한의대학교 제약공학과) | Moon-Ki Park (Department of Pharmaceutical Engineering, Daegu Haany University) Corresponding author
• 이세중(대구한의대학교 제약공학과) | Sei-Jung Lee (Department of Pharmaceutical Engineering, Daegu Haany University) Corresponding author