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참당귀 지상부 추출물의 지표성분 decursin의 분석법 개발 및 검증 KCI 등재

Development and Validation of Analytical Method for Decursin in Aerial Parts of Angelica gigas Nakai Extract

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한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)
한국식품위생안전성학회 (Korean Society of Food Hygiene and Safety)
초록

Method development and validation of decursin for the standardization of Angelica gigas Nakai as a functional ingredient and health food were accomplished. The quantitative determination method of decursin as a marker compound of aerial parts of Angelica gigas Nakai extract (AAGE) was optimized by HPLC analysis using a C18 column (3×150 mm, 3 μm) with 0.1% TFA in water and acetonitrile as the mobile phase at a flow rate of 0.5 mL/ min and detection wavelength of 330 nm. The HPLC/PDA method was applied successfully to quantification of the marker compound in AAGE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation (R2) of 0.9994 and the limit of detection and limit of quantitation were 0.011 μg/mL and 0.033 μg/mL, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 1.10% and 1.13%, respectively. Recovery of decursin at 0.5, 1, 5 and 10 μg/mL were 92.38 ~ 104.11%. These results suggest that the developed HPLC method is very useful for the determination of marker compound in AAGE to develop a health functional material.

목차
ABSTRACT
 Materials and Methods
 Results and Discussion
 국문요약
 Reference
저자
  • 김희연(강원도농업기술원 농식품연구소) | Hee-Yeon Kim (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) Correspondence to
  • 이기연(강원도농업기술원 농식품연구소) | Ki-Yeon Lee (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
  • 김태희(강원도농업기술원 농식품연구소) | Tae-Hee Kim (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
  • 박아름(강원도농업기술원 농식품연구소) | A-Reum Park (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
  • 노희선(강원도농업기술원 농식품연구소) | Hee-Sun Noh (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
  • 김시창(강원도농업기술원 농식품연구소) | Si-Chang Kim (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
  • 안문섭(강원도농업기술원 농식품연구소) | Mun-Seob Ahn (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)