The feeding habits of Pacific bluefin tuna, Thunnus orientalis were analyzed using samples by large purse seine fishery in Korean waters from 2012 to 2017. The number of samples was 1,274 and the fork-length (FL) ranged from 34.6 to 218.0 cm. According to %IRI, the Important main prey items of immature individuals (below 91.4 cm in FL) were Pisces, Cephalopoda and Euphausiacea and those of mature individuals were Cephalopoda and Pisces. T. orientalis showed ontogenetic changes in prey item that Pisces was dominated in size class of 30-89 cm (FL), Cephalopoda in 90-179 cm (FL), and Pisces above 180 cm. As for seasonal changes in prey item, Cephalopoda was dominant in spring and Pisces was dominant in summer, autumn and winter. %F, %N, %W and %IRI cluster analysis divided area into three groups: Group A was dominated by Pisces; Group B was dominated by Cephalopoda, and Group C was dominated by Euphausiacea.