In this study, the crack healing performance of concrete containing microbial - based self - healing agent for the purpose of crack crack healing was evaluated by the permeability test. As a result of the addition of the microbial culture solution to the mixed water at an alternative ratio, the permeability recovery rate increases as the substitution rate increases. It is necessary to develop a microbial concentrate and to study the application of mixed microorganisms for the purpose of increasing the rate of permeability recovery and decreasing the substitution rate of microbial solution.