This experiment was conducted to investigate the effects of pH on the mineral nutrient uptake and growth of the four Chinese chives species. The Chinese chives species used in the experiment were the cultivated species grown in the farm (cultivated Allium tuberosum) and three wild species of wild Allium tuberosum, A. thunbergii and A. senescens. The pH levels of soil medium were set to be 4.5, 6.5, and 7.5. Fresh weight (FW) of cultivated A. tuberosum was highest at all pH levels. The increase of soil pH increased the FW of the wild A. tuberosum and A. thunbergii, but no difference was noted for the A. tuberosum and A. senescens. Plant height was higher in the order of wild A. tuberosum, A. thunbergii, and cultivated A. tuberosum and A. thunbergii. Notably plant height of the wild A. tuberosum increased significantly by the pH increase. The Zn content of the wild A. tuberosum was shown to be significantly higher than that of the other species and increased with the increase of soil pH. This indicates that there is a close relationship between the plant height and Zn content in Chinese chives plant. Principal component analysis for characterizing closely related A. species using the factors of plant growth and amounts of nutrients uptake showed that the cultivated A. and wild A. tuberosum were in the 4th quadrant of the graph which are classified as the same species, while A. senescens and thunbergii was in 1st and 3rd quadrant indicating different species, respectively.