논문 상세보기
pp.148-154
The aim of this study was to select compounds for the standardization of fermented Kalopanax pictus Nakai (KP-F), to develop the analysis method using HPLC-PDA and to perform method validation. KP-F is a fermented powder developed to improve the original physiological activities and create a new functionality. Eleutheroside E, Acanthoside B, and Syringaresinol were selected as the standard compounds and developed our own method for simultaneous analysis. The analyte was isolated using C18 column with a gradient elution of 0.05 M phosphoric acid in water and methanol as the mobile phase at a flow rate of 1 mL/min and detected at 210 nm. As a result, all standard compounds showed good linearity with an R2 (coefficient of correlation) of 1.000 and for the limit of detection range of 0.710~0.831 μg/mL, and the limit of quantification as 2.150~2.520 μg/mL. The precision was RSD (%) of less than 4.80%, while the accuracy was 4.70%>RSD (%) for the range 102.44~110.48%. In conclusion, the developed analysis method is suitable for the detection of Eleutheroside E, Acanthoside B, and Syringaresinol in KP-F.
서 론
재료 및 방법
1. 실험재료 및 시약
2. 시료 제조
3. Kalopanaxsaponin 분석
4. Lignan 배당체 분석
5. 분석법 검증(Method validation)
결과 및 고찰
1. 생물전환공정에 의한 음나무발효물(KP-F)에서의 전환물질 탐색을 통한 지표성분 후보군 설정
2. 생물전환공정 진행 시간에 따른 음나무의 배당체 함량변화
3. 원산지별 음나무 및 생물전환공정에 의한 음나무발효물(KP-F)에서의 배당체 함량 조사
4. 특이성
5. 직선성 및 검출한계(LOD), 정량한계(LOQ)
6. 정밀성
7. 정확성
요약 및 결론
References
