The effects of 0.2 ppm ozone on the developing chloroplast of barley (Hordeum vulgare L.) seedling during greening were examined by chlorophyll contents, photosynthetic electron transport activity, Fo, Fv and fluorescence quenching coefficient. Chlorophyll contents of seedling treated with ozone were not changed in comparison with the control during the 96 h greening experiment, but PS Ⅱ activity of the chloroplasts of seedlings treated with ozone was decreased by 15%. Fo was slightly decreased but Fv was decreased by 13% in comparison with the control. In fluorescence quenching analysis, qP and qE were decreased by 11% and 19%, respectively, in comparison with the control. These results suggest that oxidation site of PS Ⅱ is the site affected mostly and PQ pool is also affected slightly by 0.2 ppm ozone.