We fabricated glucose oxidase (GOx)-modified biosensor for detection of glucose by physical immobilization of GOx after electrochemical polymerization of the conductive mixture monomers of the 3-thiophenecarboxylic acid (TCA) and thiophene (Th) onto ITO electrode in this study. We confirmed the successfully fabrication of GOx-modified biosensor via FT-IR spectroscopy, SEM, contact angle, and cyclic voltammetry. The fabricated biosensor has the detection limit of 0.1 μM, the linearity of 0.001-27 mM, and sensitivity of 38.75 mAM-1cm-2, respectively. The fabricated biosensor exhibits high interference effects to dopamine, ascorbic acid, and L-cysteine, respectively. From these results, the fabricated GOx-modified biosensor with long linearity and high sensitivity could be used as glucose sensor in human blood sample.

Abstract
 1. Introduction
 2. Experiments
  2.1. Reagents
  2.2. Instrumentation
  2.3. Fabrication of GOx-modifiedbiosensor after electrochemicalcopolymerization
 3. Results and Discussion
 4. Conclusions
 References

• Soo-Yeoun Kim(Department of Health Cosmetic Science, Dongduk Women's University)
• Hyeon-Jin Jo(Department of Chemistry, Hannam University)
• Seong-Ho Choi(Department of Chemistry, Hannam University) Corresponding author