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Evaluation of the adjuvants effect on the immunoglobulin class switching in generation of monoclonal antibodies against Paenibacillus larvae KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

American foulbrood (AFB) is caused by the bacterium Paenibacillus larvae, which is highly contagious and often lethal to honeybee broods. To control AFB, rapid diagnostic tools including those based on immunological methods are required. We produced several specific mouse monoclonal antibodies (MAbs) against P. larvae. Interestingly, a few of the MAbs were revealed to be an IgM-type antibody. To ascertain the effects of adjuvants on immunoglobulin isotype switching, BALB/c mice were immunized with various adjuvants, i.e., Freund's adjuvant (FA), Alum adjuvant, and AddaVax™ followed by the generation of hybridoma that secreted monoclonal antibodies to P. larvae. In the case of AddaVax™, all screened hybridoma clones secreted IgG-type MAbs, whereas hybridomas generated by Alum and FA secreted 91.25% (7/80) and 66.67% (11/33) respectively, IgG-type MAbs. Although the mechanism of incomplete immunoglobulin isotype switching associated with the P. larvae antigen needs further study, our results indicate that the applied adjuvants can have a significant effect on immunoglobulin isotype switching results.

목차
Abstract
 서 론
 재료 및 방법
  P. larvae 면역원
  Balb/c Mouse 면역
  Immunoglobulin isotype 확인 ELISA
  단클론 항체의 Immunoglobulin isotype 검사
 결 과
  Stock Mab clones의 Isotype
  Adjuvant에 따른 면역마우스 혈청 Immunoglobulin의항체가 비교
  Adjuvant별로 비교한 MAb의 Isotype
 고 찰
 REFERENCES
저자
  • Jeong-Ah Yu(College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • Suyeon Kim(College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • Eun-Ju Ko(Vaccine Branch, National Cancer Institute, National Institutes of Health)
  • Hong-Gu Joo(College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
  • Jaehyuk Yang(Department of equine industry, Korea National College of Agriculture and Fisheries)
  • Yoon-Kyu Lim(College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) Corresponding Author