Prestress is a reinforcement method to control crack due to moment on concrete girders with low tensile strength. In the existing literature, it is mentioned that prestress for ordinary concrete affects not only crack control but also shear strength enhancement. As the construction material improves, UHPC(Ultra-HIgh Performance Concrete) with excellent strength and ductility has been developed by combining ultra-high strength concrete and steel fiber. However, study on the effect of prestress on the shear strength of UHPC with different material properties from ordinary concrete is lacking. Therefore, in this paper, the effect of prestress on the shear behavior of UHPC I-girder is studied by finite element analysis. As a result of the analysis, it has been confirmed that the prestress increases crack strength and shear strength of UHPC.