In this paper, we investigate to determine quality characteristics, fatty acid composition and cytotoxic effect of extracts and fractions from whole Lycopus lucidus Turcz. roots. Additionally, we evaluated cytotoxic activity against the growth of human fibrosarcoma cells (HT-1080) and human gastric adenocarcinoma (AGS), human colon cancer cell (HT-29) lines using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Acetone+methylene chloride (A+M) and methanol (MeOH) extracts from L. lucidus Turcz. were obtained through solvent extraction. Then we further fractionated both extracts with n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water. In fatty acid composition, L. lucidus Turcz. contained 33.2% of 18:1n-9 and 1.81% of 18:3n-3, respectively. The incorporation of treatment with A+M and MeOH extracts and n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water fractions dose-dependently increased cytotoxicity against the growth of HT-1080 and AGS, HT-29 cancer cells (p<0.05). The A+M extract had a higher inhibitory effect on the growth of all cancer cells in comparison to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions showed a higher inhibitory effect after proliferating the three cancer cells. These results suggest that the 85% aq. MeOH and n-hexane fractions have a potential to inhibit the growth of human cancer cell lines.

Abstract
 서 론
 재료 및 방법
  1. 재료
  2. 추출 및 분획
  3. 일반성분 분석
  4. 지질 및 지방산 추출
  5. Gas chromatography를 이용한 지방산 분석
  6. MTT assay
  7. 통계분석
 결과 및 고찰
  1. 택란 일반성분 및 지방산 조성
  2. 택란 추출물 및 분획물의 세포독성 효과
 요약 및 결론
 References

• 나은(한국해양대학교 해양과학기술전문대학원) | Eun Na (Ocean Science and Technology School, Korea Maritime & Ocean University)
• 이정우(한국해양대학교 해양생명과학부) | Jung-Woo Lee (Division of Marine Bioscience, Korea Maritime & Ocean University)
• 임선영(한국해양대학교 해양생명과학부) | Sun-Young Lim (Division of Marine Bioscience, Korea Maritime & Ocean University) Corresponding author