논문 상세보기
pp.59-68
Low molecular weight hydrolysates from donkey bone extracts (LHDB) was prepared with different food enzymes, and its antioxidative, elastase and collagenase inhibitory, and fibroblast cell protection effects against photoaging were evaluated. Gelatin from donkey bone was extracted three times at 121℃ for 1 h and was lyophilized. The lyophilized powder (5 g) was dissolved in 95 mL distilled water with 1% FoodPro alkaline protease (A), 1% Protease P (P), 1% Protease M (M) and a 0.3% A + 0.3% P + 0.3% M (APM) mixture and was hydrolyzed for 3 h at 45℃. After enzyme inactivation at 90℃ for 10 min, the LHDBs hydrolyzed by A, P, M, and APM were separated by centrifugal filtration and were lyophilized and marked as LHDB-A, LHDB-P, LHDB-M, LHDB-APM. The LHDB-M showed higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline–6- sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) than the other treatments (p<0.05). The elastase inhibition effect (37.49%) of LHDB-M were significantly higher than those of the other treatments (9.97-34.18%). The viability of human fibroblast cells (Hs68) after UVB irradiation was significantly increased by LHDB-M, indicating that it can be used as an antioxidant or as a UVB stress protector. However, further in vivo studies should precede its usage in the bioactive compound industry.
Ⅰ. 서론
Ⅱ. 재료 및 방법
1. 시료의 제조
2. 가수분해도
3. 항산화 활성
4. Elastase 저해 활성
5. Collagenase 저해 활성
6. MALDI-TOF를 이용한 당나귀 사골 저분자 추출물의분자량확인
7. 세포배양 및 자외선(UVB) 조사
8. 세포독성 및 생존율 측정
9. 통계분석
Ⅲ. 결과 및 고찰
1. 가수분해도
2. 항산화 활성
3. Elastase와 collagenase 억제효과
4. 당나귀 사골 저분자 추출물의 분자량확인
5. 세포독성 및 자외선 조사 후 생존율
Ⅳ. 요약
Ⅴ. 참고문헌
