In this study, the performances of the refrigeration unit for the box under actual operating conditions were examined by using the constant temperature and humidity chamber (air enthalpy method). For this purpose, the degree of superheat, pressure, and temperature were measured while changing the EEV opening, the compressor speed, the outdoor temperature, and the load in the refrigeration cycle of the refrigeration unit. The highest cooling performance was found at EEV 33%. However, EEV 35% with low compression ratio and degree of superheat was the most reliable in terms of reliability. Considering the compressor speed and cooling performance, the refrigeration unit was in an optimal condition at the compressor speed 3,040RPM. The results of this study will be provided as basic data for the optimal design of refrigeration units for box.