Mesenchymal stem cells (MSCs) are multipotent cells capable of replicating as undifferentiated cells, and have the potential of differentiating into mesodermal lineages. Goats are commonly used as animal models for bone tissue engineering to test the potential of stem cells for bone regeneration. Goat MSCs isolated from bone marrow (BM) or adipose tissue (AD) should be evaluated using in vitro assays, prior to their application in a tissue engineering project. In this study, we compared the stem cell properties of MSCs derived from goat AD, BM and ear skin tissue (ESK). As results, BM and ESK-MSCs exhibited a spindle-shaped morphology comparable to that of AD-MSCs. Especially, BM-MSCs could be cultured for significantly longer periods and exhibited the greatest expansion capacity, whereas AD-MSCs had the shortest culture time and lowest growth rate. Also, we compared differentiation potentials of AD, BM and ESK-MSCs into adipogenic, chondrogenic, and osteogenic lineages through specific staining and quantitative real-time RT-PCR. Collectively, we successfully isolated ESK-MSCs from goat for the first time. This study suggests that adult skin tissue of goat could be used as a source of goat MSCs. Further studies are needed to show the more information for establishment and fully characterization of goat ESK-MSCs.

Abstract
서 론
재료 및 방법
    염소 유래 성체줄기세포 분리 및 배양
    세포 증식능 분석(cumulative population doubling levelanalysis, CPDL)
    정량적 중합효소연쇄반응(quantitative real-time RT-PCR)을 이용한 유전자 분석
    염소 유래 성체줄기세포의 중배엽세포 분화
    통계학적 분석
결 과
    염소 유래 성체줄기세포의 성상 및 증식능 비교
    염소 유래 성체줄기세포 특이마커 발현 비교
    염소 유래 성체줄기세포의 중배엽세포로의 분화능 비교
고 찰
REFERENCES

• Na-Yeon Gu(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Da-Un Jeong(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Jeong Su Byeon(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Jae-Young Song(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• In-Soo Cho(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Bang-Hun Hyun(Viral Disease Research Division, Animal and Plant Quarantine Agency)
• Jienny Lee(Viral Disease Research Division, Animal and Plant Quarantine Agency) Corresponding Author.