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Isolation and characterization of deer-derived mesenchymal stem cells KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Mesenchymal stem cells (MSCs) are capable of differentiating into mesenchymal tissue such as bone, cartilage, muscle, and adipose, and have been isolated and characterized from various species. Deer adipose tissue-derived MSCs (dAD-MSCs) have not been studied and deer bone marrow-derived MSCs (dBM-MSCs) have not been fully characterized. In this study, we firstly isolated MSCs from deer tissues and then compared characteristics of dAD-MSCs and dBM-MSCs. dAD-MSCs and dBM-MSCs exhibited significant increase in proliferation under low-glucose DMEM culture condition during 20 and 10 passages consecutive passages, respectively. Both cells expressed cell surface markers such as CD73, CD90, and CD105, but did not express CD34 and CD45. Two types of cells expressed stemness markers (Oct4, Sox2, and Nanog) and exhibited differentiation potential into mesodermal lineages. Both cells exhibited osteogenic and chondrogenic differentiation potential, but poor adipogenic differentiation potential. Specifically, dAD-MSCs have a greater capacity for chondrogenic differentiation potential compared to dBM-MSCs. Collectively, we successfully isolated dAD-MSCs from deer for the first time. This study suggests that adipose tissue of deer could be used as a source of deer MSCs.

목차
Abstract
서 론
재료 및 방법
    사슴 지방조직 및 골수 유래 성체줄기세포 분리 및 배양
    세포 증식능 분석(cumulative population doubling levelanalysis, CPDL)
    정량적 중합효소연쇄반응(quantitative real-time RT-PCR)을 이용한 유전자 분석
    사슴 유래 성체줄기세포의 중배엽세포 분화 유도 및 분화능 분석
    통계학적 분석
결 과
    사슴 지방조직 및 골수 유래 성체줄기세포의 성상 및 증식능 비교
    사슴 지방조직 및 골수 유래 성체줄기세포 세포표면 단백질 및 초기전사인자 발현 비교
    사슴 지방조직 및 골수 유래 성체줄기세포의 중배엽세포 분화능 비교
고 찰
REFERENCES
저자
  • Jeong Su Byeon(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Na-Yeon Gu(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Da-Un Jeong(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Chan-Lan Kim(Animal Genetic Resources Research Center, National Institute of Animal Science)
  • In-Soo Cho(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Jae-Young Song(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Bang-Hun Hyun(Viral Disease Research Division, Animal and Plant Quarantine Agency)
  • Jienny Lee(Viral Disease Research Division, Animal and Plant Quarantine Agency) Corresponding Author.