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사료 접종 방법에 의한 in vitro 반추위 발효 상성 변화 KCI 등재

Rumen Fermentation was Changed by Feed Inoculation Method in In Vitro

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동물자원연구 (Annals of Animal Resources Sciences)
강원대학교 동물자원공동연구소 (Institute of Animal Resources Kangwon National University)
초록

The objective of this study was to investigate the effect of different feed inoculation method on rumen fermentation in an in vitro. Three experimental treatments were used: control (CON, direct dispersion of feed (2 g) in rumen fluid), combinations of direct dispersion (1 g) and nylon bag (DNB, pore size: 50 μm, 1 g), and nylon bag (NB, 2 g). An in vitro fermentation experiment was carried out using strained rumen fluid for 48 h incubation time and timothy was used as a substrate. At the end of the incubation, in vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), pH, volatile fatty acids (VFA), ammonia nitrogen (NH3-N), and microbial community were evaluated and gas production was estimated at 3, 6, 12, 24, 48 h incubation periods. Gas production was higher in CON than DNB and NB at 6 and 12 h incubation time (p<0.01). There were no differences in final gas production, pH, NH3-N concentration, total VFA production, and VFA profiles among treatments. The IVDMD was lowest in CON (p<0.01) but the IVNDFD was not differed by feed distribution methods. There were no significant differences in general bacteria and fungi. Protozoa count was highest in NB treatment among treatments (p<0.01). The abundance of cellulolytic bacteria, Ruminococcus flavefaciens and Fibrobacter succinogenes, was highest in the CON among treatments (p<0.01).

목차
ABSTRACT
Ⅰ. 서론
Ⅱ. 재료 및 방법
    1. 공시재료 및 공시동물
    2. 영양소 성분분석
    3. In vitro 발효 평가
    4. genomic DNA 추출
    5. 재조합 plasmid의 제작
    6. Real-time PCR
    7. Gas parameter
    8. 통계 분석
Ⅲ. 결과 및 고찰
Ⅳ. 요약
Ⅴ. 참고문헌
저자
  • 유대겸(부산대학교 생명자원과학대학 동물생명자원과학과) | Dae-Kyum Yoo (Life and Industry Convergence Research Institute, Department of Animal Science, Pusan National University)
  • 문준범(부산대학교 생명자원과학대학 동물생명자원과학과) | Joon-Beom Moon (Life and Industry Convergence Research Institute, Department of Animal Science, Pusan National University)
  • 김한빈(부산대학교 생명자원과학대학 동물생명자원과학과) | Han-Been Kim (Life and Industry Convergence Research Institute, Department of Animal Science, Pusan National University)
  • 양성재(부산대학교 생명자원과학대학 동물생명자원과학과) | Sung-Jae Yang (Life and Industry Convergence Research Institute, Department of Animal Science, Pusan National University)
  • 박중국(농협사료) | Joong-Kook Park (Nonghyupfeed Co., Ltd)
  • 이세영(천안연암대학교 축산계열) | Se-Young Lee (Department of Animal Science, Yonam College)
  • 서자겸(부산대학교 생명자원과학대학 동물생명자원과학과) | Ja-Kyeom Seo (Life and Industry Convergence Research Institute, Department of Animal Science, Pusan National University) Corresponding Author