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pp.511-521
The purpose of this study was to investigate the inhibitory effect of enzyme activity and anti-proliferation of human cancer cell lines (HCT 116, NCI-H460 and MCF-7) of peanut skin depending on cultivars (Arachis hypogaea L. cv. K-Ol, cv. Sinpalkwang, cv. Daan, cv. Heuksaeng) and extraction solvent. Peanut skin was extracted with 80% ethanol, 80% methanol, 80% acetone, and distilled water, followed by analysis of the enzyme inhibitory activity and anticancer activity. Methanol extract of Daan cultivar most effectively inhibited α-gluosidase (65.08%, 0.025 mg/mL), tyrosinase (82.49%, 2 mg/mL) and ACE (73.61%, 10 mg/mL). The inhibitory effect of peanut skin extracts on colon cancer cell (HCT-116), lung cancer cell (NCI-H460) and breast cancer cell (MCF-7) growth were investigate using MTT assay. The highest anti-proliferation of cancer cell line of peanut skin extracts was observed in the methanol extract of Daan cultivar. The cell viability on HCT 116, NCI-H460 and MCF-7 cell lines of methanol extracts from peanut skin of Daan cultivar was 48.13%, 41.03%, and 36.02% at 200 μg/mL, respectively. These results suggest that peanut skin extracts may mediate physiological activity, and provide valuable information for the use of peanut byproduct as a functional food material.
서 론
재료 및 방법
1. 실험재료 및 추출물 제조
2. α-Glucosidase 저해활성 측정
3. Tyrosinase 저해활성 측정
4. Angiotensin converting enzyme I (ACE) 저해활성 측정
5. 암세포주 배양
6. 세포독성 측정
7. 암세포주 성장억제 효과 측정
8. 통계분석
결과 및 고찰
1. α-Glucosidase 저해활성
2. Tyrosinase 저해활성
3. Angiotensin converting enzyme I (ACE) 저해활성
4. 폐암 (NCI-H460) 성장억제효과
5. 대장암세포(HCT-116) 성장억제효과
6. 유방암세포(MCF-7) 성장억제효과
7. 상관관계분석
요약 및 결론
References
