Proteasome inhibitors can improve the efficiency of cancer treatments by inhibiting nuclear factor κB(NF-κB) activation in cancer cells. Lentils are a type of beans of which consumption of such beans is increasing. The purpose of this study was to investigate the effects of lentils extract (LE) on the proteasomal activities, NF-κB activation, and cell cycle in HepG2 human liver cancer cells. LE treatments inhibited proteasomal activities at concentrations of 10, 50, and 100 μg/mL respectively, and repressed NF-κB activation at concentrations of 1, 10, and 100 μg/mL respectively, in HepG2 cells. LE treatments at concentrations of 1, 10, and 100 μg/mL respectively, increased sub-G1 cell population in HepG2 cells, which may be the result of apoptosis. The results suggest that LE inhibited NF-κB activation partially with its proteasome inhibitory activities, and the increase of sub-G1 cell population was induced partially, by inhibition of NF-κB activation in HepG2 cells.

Abstract
서 론
재료 및 방법
    1. 재료
    2. 렌즈콩 추출물 제조
    3. 세포 배양
    4. 세포 독성 측정
    5. Proteasome 억제 활성 측정
    6. NF-κB 활성화 측정
    7. Cell cycle 분석
    8. 통계처리
결과 및 고찰
    1. 렌즈콩 추출물의 세포 독성
    2. 렌즈콩 추출물의 proteasome 억제 활성
    3. 렌즈콩 추출물이 NF-κB 활성화에 미치는 영향
    4. 렌즈콩추출물이HepG2 세포의cell cycle에미치는영향
요약 및 결론
References

• 민수영(성신여자대학교 식품영양학과) | Sooyeong Min (Dept. of Food and Nutrition, Sungshin Women’s University)
• 윤현근(성신여자대학교 바이오식품공학과) | Hyungeun Yoon (Dept. of Food Science and Biotechnology, Sungshin Women’s University) Corresponding author