Experiments were conducted on the operating characteristics and performance of various types of working fluid, filling amount and heat flow rate of a loop thermosyphon for cooling ESS battery container. As results of performance test on various working fluids, HFE-7100 and R-134a as a working fluids showed unstable operating and low performance due to vapor pressure drop, and performance was improved by increasing the number of vapor lines for reducing a pressure drop. In this study, n-pentane was more stable and showed better thermal performance among various working fluids.