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한지형 마늘 생장점 배양체의 세대별 바이러스 감염양상과 생육특성 KCI 등재

Virus Infection and Growth of Advanced Generations in Northern-type Garlic bulbs from Meristem Culture

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한국국제농업개발학회지 (The Journal of the Korean Society of International Agriculture)
한국국제농업개발학회 (The Korean Society Of International Agriculture)
초록

1. 경북지역 한지형 마늘 주산지인 의성지역을 중심으로 OYDV, LYSV, GCLV, SLV 그리고 Allexivirus를 진단한 결과, 모든 마늘잎의 시료가 한 가지 이상의 바이러스에 복합감염되어 있었다..
2. 생장점 배양한 마늘은 1세대부터 3세대까지 OYDV와 SLV는 전혀 검출되지 않았다. 생장점 배양 마늘의 1세대는 GCLV 3.8%, Allexivirus 3.1%의 바이러스 감염률을 나타내었으며, 생장점 배양 마늘 2세대는 LYSV 3.4%, GCLV 21.7% 그리고 Allexivirus 10.0%를 나타내었다. 생장점 배양 마늘 3 세대는 GCLV 17.5%, Allexivirus 7.5%의 바이러스 감염률을 나타내었다.
3. 의성지역의 바이러스에 감염된 일반마늘 1통의 평균 무게는 29.3 g 이었으나, 생장점 배양에 의하여 증식된 2세대 마늘 1통의 평균 무게는 57.6 g, 3세대 마늘 1통의 평균 무게는 66.2 g 이었다.

All conventional garlics cultivated at farmer’s fields were infected with various plant viruses such as Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV) and Allexivirus. The infection rate of these viruses in northern-type garlics was from 14% to 100% in Uiseong and Yecheon area. For producing virus-free garlic, meristem was removed from garlic plants and cultivated in Murashige & Skoog (MS) medium. No OYDV and Shallot latent virus (SLV) were detected on leaf of garlic from first generation to 3rd generation. Virus infection rates of 2nd generation of garlic plants grown in net houses were 3.4%, 21.7% and 10.0% for LYSV, GCLV and Allexivirus, respectively, and those of 3rd generation were 17.5% and 7.5% for GCLV and Allexivirus, respectively. The average weight of a bulb infected with viruses in Uiseong was 29.3 g but that of bulbs from meristem culture in 2nd and 3rd generations were 57.6 g 66.2 g, respectively, showing more than two times increase in bulb weight.

목차
ABSTRACT
서 론
재료 및 방법
    마늘재배지 시료채취 및 바이러스 진단
    마늘 생장점 적출
    마늘 생장점 배양
    생장점 배양 마늘의 재배와 조사
결과 및 고찰
    마늘 재배현장의 바이러스 감염실태
    마늘 조직배양
    생장점 배양 마늘의 세대별 바이러스 감염 양상
    생장점 배양 마늘의 세대별 생육
적 요
REFERENCES
저자
  • 정원권(경북농업기술원 유기농업연구소) | Won-Kwon Jung (Organic Agricultural Research Institute, Gyeongsangbuk-do Agricultural Research & Extension Services) Corresponding author
  • 이지은(경북농업기술원 원예경영연구과) | Ji-Eun Lee (Department of Horticulture & Management Research Department, Gyeongsangbuk-do Agricultural Research & Extension Services)
  • 이문중(경북농업기술원 원예경영연구과) | Mun-Jung Lee (Department of Horticulture & Management Research Department, Gyeongsangbuk-do Agricultural Research & Extension Services)
  • 도한우(경북농업기술원 유기농업연구소) | Han-Woo Do (Organic Agricultural Research Institute, Gyeongsangbuk-do Agricultural Research & Extension Services)
  • 연일권(경북농업기술원 유기농업연구소) | Il-Kweon Yeon (Organic Agricultural Research Institute, Gyeongsangbuk-do Agricultural Research & Extension Services)
  • 박석희(경북농업기술원 유기농업연구소) | Seuk-Hee Park (Organic Agricultural Research Institute, Gyeongsangbuk-do Agricultural Research & Extension Services)