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pp.149-158
The purpose of this study was to investigate antioxidant characteristics and anti-adipogenic effects of colored rice and brown rice extracts in 3T3-L1 adipocyte depending on cultivar (Josaengheugchal, Heugjinmi, Hongjinju, Geongganghongmi, Seolgaeng, Milyang 320, Sindongjin, Baegjinju). Colored rice and brown rice was extracted with 100% ethanol, followed by the analysis of polyphenol, flavonoid, anthocyanin, antioxidant, and anti-adipogenic activity. Total polyphenol and flavonoid content ranged from 6.86~314.08 mg GAE/g and 1.47~56.88 mg CE/g the highest total polyphenol and flavonoid content was observed in Heugjinmi cultivar. Anthocyanin composition was analyzed by HPLC, cyanidin-3-gluoside and peonidin-3-glucoside was found in black rice including Josaengheugchal and Heugjinmi. Also, the ABTS and DPPH radical scavenging activity of colored rice cultivars was higher than that of brown rice cultivars, the highest ABTS radical scavenging activity also was observed in Heugjinmi (128.20 mg TE/g). The anti-adipogenic effects of colored rice and brown rice extracts on differentiation of 3T3-L1 preadipocytes evaluated that extracts of Heugjinmi cultivar significantly reduced intracellular lipid accumulation. These results provide valuable information for the use of Korean colored rice cultivar as a functional food materials relative to anti-obesity.
서 론
재료 및 방법
1. 실험재료 및 추출물 제조
2. 총 폴리페놀 함량 측정
3. 총 플라보노이드 함량 측정
4. 안토시아닌 함량 분석
5. ABTS 라디칼 소거능 측정
6. DPPH 라디칼 소거능 측정
7. 세포배양 및 독성 측정
8. 3T3-L1 preadipocyte의 분화 유도
9. Oil Red O staining
10. 통계분석
결과 및 고찰
1. 총 폴리페놀 및 플라보노이드 함량
2. 안토시아닌 함량
3. 항산화 활성
4. 세포 내 지방축적 억제 효과
5. 상관관계 분석
요약 및 결론
References
