The purpose of this study was to investigate the protective effect on oxidative stress induced PC12 cells, and volatile flavor composition of essential oils derived from medicinal plant seeds- Gossypium hirsutum L. (G. hirsutum), Coix lachryma-jobi (C. lachryma-jobi) and Oenothera biennis (O. biennis). The essential oils were obtained by the solvent (hexane) extraction method from the seeds. The essential oils of the seeds were analyzed by the solid-phase micro-extraction gas chromatography mass spectrometry (SPME-GC/MS). The major compounds of G. hirsutum, C. lachryma-jobi and O. biennis were cyclonexanol (16.65%), β-asarone (14.29%) and ylangene (50.01%). The DPPH radical scavenging activity (IC50) was the highest value of 8.52 mg/mL in the O. biennis. Additionally, IC50 values of G. hirsutum and C. lachryma-jobi were 26.76 mg/mL and 36.81 mg/mL. For the oxidative stress on PC12 cells, we treated with hydrogen peroxide (H2O2). The pretreatment of oxidative stress induced PC12 cells with all the essential oils preserved or increased their cell viability and G. hirsutum and O. biennis attenuated the ROS generation (by 68.75% and 56.25% vs. H2O2 control). The results of this study suggest that the essential oils derived from medicinal plant seeds could be used as valuable back data as a natural essential oil material to prevent neurodegenerative diseases by protecting neuro-cells.

Abstract
서 론
재료 및 방법
    1. 추출물 준비
    2. 2,2-Diphenyl-1-picrylhydrazyl(DPPH) 라디칼 소거능 측정
    3. 세포 배양
    4. 세포 생존율 측정
    5. 세포 내 ROS 측정
    6. GC/MS 분석 조건
    7. 통계 처리
결과 및 고찰
    1. 약용식물유래정유추출물의수율및항산화활성평가
    2. 약용식물 유래 정유 추출물의 신경세포 보호 효과
    3. 약용식물 유래 정유 추출물의 성분 분석
요약 및 결론
References

• 이지연(충북대학교 식품공학과) | Ji Yeon Lee (Department of Food Science and Biotechnology, Chungbuk National University)
• 박정용(농촌진흥청 국립원예특작과학원 인삼특작부) | Jeong-Yong Park (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
• 김동휘(농촌진흥청 국립원예특작과학원 인삼특작부) | Dong Hwi Kim (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
• 최수지(농촌진흥청 국립원예특작과학원 인삼특작부) | Su Ji Choi (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
• 장귀영(농촌진흥청 국립원예특작과학원 인삼특작부) | Gwi Young Jang (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
• 서경혜(농촌진흥청 국립원예특작과학원 인삼특작부) | Kyung Hye Seo (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) Corresponding author