The variation of instrumental profile for the different scan speed of PDS is estimated as FWHM of the assumed Gaussian Profile. The effects of scan speed and scan aperture of PDS on the objective prism spectrum analysis are investigated for 8 combinations of scan speed and scan aperture. Amomg them. D1 apture with 15 csu is found to be the most optimum choice for measuring KISO objective prism film. We suggest the preliminary test study of the scan speed and aperture for the optimum use of PDS for any massive scan of spectra. The optimum scan speed and aperture depends on the dispersion of spectrum as well as the type of phtographic emulsion.