Cognitive impairment is considered to be key research topics in the field of neurodegenerative diseases and in understanding of learning and memory. In the present study, we investigated neuroprotective effects of Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts in hydrogen peroxide-induced neuronal cell death in vitro and scopolamine-induced cognitive impairment in Sprague Dawley® (SD) rat in vivo. Apoptotic cell death in neuroblastic PC12 cell line was induced by hydrogen peroxide for 1 hour at 100 μM. However, mixture of SC and RF treatment prevented peroxide induced PC12 cell death with no neurotoxic effects. For in vivo experiment, the effect of SC and RF extracts on scopolamine-induced cognitive impairment in SD rat was evaluated by spontaneous alternation behavior in Y-Maze test. After 30 min scopolamine injection, the scopolamine-induced rats presented significantly decreased % spontaneous alteration and acetylcholine level, compared to non-induced group. However, treatment of SC+RF extracts rescued the reduced % spontaneous alteration with acetylcholine concentration from hippocampus in scopolamineinduced rats. These results suggested that mixture of SC and RF extract may be a potential natural therapeutic agent for the prevention of cognitive impairment.

Abstract
서 론
재료 및 방법
    1. 시료 제조
    2. 신경세포 배양
    3. 세포 독성 평가
    4. Annexin-propidium iodide(PI) 유세포 분석
    5. 단백질 추출 및 단백질 분석
    6. 실험동물 사육 및 실험설계
    7. Y-maze 실험
    8. 해마의 acetylcholine(Ach) 농도 측정
    9. 통계처리
결과 및 고찰
    1. PC12 세포에서 과산화수소(H2O2)의 처리에 따른 독성평가
    2. PC12세포에서 오미자칠해목 추출혼합물의 독성 및보호효과 확인
    3. 오미자칠해목 추출혼합물 처리에 의한 세포사 확인
    4. Y-maze 실험
    5. 해마의 acetylcholine(Ach) 농도 측정
요약 및 결론
References

• 박은국(아주대학교 의과대학 의학유전학과) | Eun-kuk Park (Dept. of Medical Genetics, Ajou University School of Medicine)
• 한경훈(서울의료원 의학연구소) | Kyung-Hoon Han (Research Institute, Seoul Medical Center)
• 허재혁(서울의료원 신경과) | Jae-Hyeok Heo (Dept. of Neurology, Seoul Medical Center)
• 김남기(알피바이오㈜ 연구소) | Nam-Ki Kim (Rpbio Research Institute, Rpbio Co. Ltd)
• 배문형(알피바이오㈜ 연구소) | Mun-Hyoung Bae (Rpbio Research Institute, Rpbio Co. Ltd)
• 서영하(알피바이오㈜ 연구소) | Young-Ha Seo (Rpbio Research Institute, Rpbio Co. Ltd)
• 용윤중(㈜나인비 연구소) | Yoon-joong Yong (NineB Research Institute, NineB Co. Ltd)
• 정선용(㈜나인비) | Seon-Yong Jeong (NineB Co. Ltd)
• 최춘환((재)경기도경제과학진흥원 바이오센터 천연물연구팀) | Chun-Whan Choi (Natural Product Research Team, Biocenter, Gyeonggido Business and Science Accelerator) Corresponding author