Osteoarthritis is a disorder characterized by a loss of cartilage as common aging-associated disease in humans and animals. However, unlike human clinical trials, investigational studies in pet animals are constrained by a lack of interest and funds. In addition, pet owners would often prefer the lowest cost method to treat arthritis of pet animals. Here, we report the outstanding and inexpensive way to prepare chondrocytes for cartilage repair using rabbit adipose derived mesenchymal stem cells (MSCs). This study focused on the development and enhancement of pre-chondrogenic condensation under external electric fields even without additional growth factors. We found that highly compact structures were formed within 3 days in micromass cultures of rabbit MSCs under electrical stimulation (ES), showing increased COL2A1 gene expression compared with their control 3D micromass cultures and 2D monolayer cultures. We further found that ES enhanced the production of proteoglycan, a highly produced extracellular matrix component in chondrocytes. Collectively, these results provide the commercial potential of electrical stimulation driving chondrogenesis of mesenchymal stem cells for repair of cartilage, which is a budget-friendly regimen.

Abstract
서 론
재료 및 방법
    지방유래 중간엽 줄기세포의 분리 및 배양
    Micromass의 제작 및 실험관내 전기자극법
    실시간 중합효소 연쇄반응 검사 (Real-time PolymeraseChain Reaction)
    Alcian Blue 염색
결 과
    토끼의 지방 유래 중간엽 줄기세포의 배양
    전기 자극에 의한 micromass의 제작
    전기 자극 응집체의 연골 분화 관찰
    전기자극 응집체의 세포 외 기질 생성
고 찰
REFERENCES

• Chi Young Chang(Bio Biz Division, Hanyang Digitech)
• Juhyun Park(Bio Biz Division, Hanyang Digitech)
• Kisuk Kim(Bio Biz Division, Hanyang Digitech) Corresponding Author.