The evergreen oak tree, Quercus myrsinaefolia Blume, is not only economically important for wood, medicine, landscape trees, etc., but also becoming more important in terms of ecology due to climate change. However, asexual reproduction was difficult, so this study was conducted to establish the optimum conditions for micropropagation by shoot multiplication. The surface sterilized seeds of Q. myrsinaefolia were successfully germinated in WPM basal medium. BAP (1.0 mg/L) treatment was most effective for inducing multiple shoots. The highest induction rates of adventitious roots from the multiple shoots was shown in the treatment of 1.0 mg/L NAA. Both MS and WPM medium were most effective for growth of multiplied plantlets. For ex vitro acclimatization, the survival rates of multiplied plantlets were 100% in vermiculite and commercial soil. The results of this study can be used for proliferation and supply, and establishment of ex situ conservation of Q. myrsinaefolia elite trees.

Abstract
Introduction
Materials and Methods
Results
Discussion
References

• Dong-Jin Park(Department of Seed and Seedling Management, National Forest Seed and Variety Center)
• Seong-Hyeon Yong(Division of Environmental Forest Science, Gyeongsang National University)
• Myung-Suk Choi(Division of Environmental Forest Science, Gyeongsang National University/Institute of agriculture and Life Science, Gyeongsang National University, Jinju) Corresponding author