The fatty acid binding protein 4 (FABP4) gene plays an important role in lipid metabolism and homeostasis in adipocytes. The objective of this study was to analyze the association between a single nucleotide polymorphism (SNP), g.7516G>C, in the FABP4 gene and economic traits of Korean native cattle, Hanwoo. Primers were designed to target a region of the FABP4 gene between nucleotides 7417 and 7868 (AAFC01136716). The SNP, which was detected by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method using restriction enzyme MspA1I, was genotyped in 319 animals of Hanwoo steer population. Statistical analysis showed that the SNP genotype of the FABP4 gene significantly affected carcass weight (CW, p<0.01), longissimus muscle area (LMA, p<0.001), and marbling score (MS, p<0.001). GG allele of the SNP on 246 animals in a Wagyu × Limousin F2 reference population showed a higher MS (p<0.05) and subcutaneous fat depth (p<0.05) in previous report. But CC allele of the SNP showed greater values for MS, LMA, and CW in Hanwoo steers. These results suggest that the g.7516G>C SNP located in the FABP4 gene may affected differently depending on the cattle breed and can be used as a genetic selection marker in Korean native cattle.

ABSTRACT
Ⅰ. Introduction
Ⅱ. Materials and Methods
    1. Animals and data collection
    2. DNA extraction
    3. PCR-RFLP genotypes
    4. Statistical analysis
Ⅲ. Results and discussion
Ⅳ. References

• 이승규(강원대학교 동물생명과학대학) | Seung-Kyoo Lee (College of Animal Life Sciences, Kangwon National University)
• 이영섭(강원대학교 동물생명과학대학) | Young-Sub Lee (College of Animal Life Sciences, Kangwon National University)
• 김훈(강원대학교 동물생명과학대학) | Hun Kim (College of Animal Life Sciences, Kangwon National University)
• 배윤호(강원대학교 동물생명과학대학) | Yun-Ho Bae (College of Animal Life Sciences, Kangwon National University)
• 이성진(강원대학교 동물생명과학대학) | Sung-Jin Lee (College of Animal Life Sciences, Kangwon National University) Corresponding author