Salmonella Enteritidis (SE) infection, one of a major foodborne disease remains considerable public health concerns globally. In this study, we have constructed that the genetically inactivated SE ghost vaccine candidate, JOL2220 (Δlon ΔcpxR Δasd), which express the immunogenic hemagglutinin portion (HA1) of H1N1 virus on the Salmonella surface. The lysis gene cassette, holin-endolysin system originated from double DNA bacteriophage λ was cloned into the ghost vector plasmid pJHL464, which activated under the control of the convergent promoter system. Scanning electronic microscopic examination revealed that transmembrane tunnels of JOL2220 was formed following the 48 hr of the lysis mediated by the holin-endolysin system. In vitro expression of HA1 antigenic protein was also determined by Western blot analysis. The Chickens immunized with JOL2220 ghost via intramuscular and oral routes showed significant increase in IgG level against both SE and HA1 antigen. Further, we observed markedly enhancement of lymphocyte proliferation and T-cell differentiation in the primed PBMC of the immunized chicken, compared to those in the control group. Collectively, the results indicated that the SE ghosts expressing HA1 antigen may have immunostimulatory properties against Salmonella and influenza infection where the chicken is the predominant reservoir.

Abstract
서 론
재료 및 방법
    HA1 항원단백질 발현 살모넬라 백신 후보 균주의 제작
    백신 후보 주 면역원성 평가를 위한 동물실험
    면역화된 산란계의 체액성 면역반응
    면역화된 산란계의 세포성 면역 반응 측정
    MTT 세포 생존능 분석 및 면역 사이토카인 분석
    유세포 분석
    면역 사이토카인 분석
    수지상 세포의 분화 측정
    통계분석
결 과
    백신 후보주 JOL2220 고스트 균주의 제작 및 HA1 외래항원 단백질의 발현
    체액성 면역원성 의 확인
    세포매개성 면역원성 측정
    항원자극에 의한 수지상 세포의 분화 측정
고 찰
REFERENCES

• Gayeon Won(College of Veterinary Medicine, Jeonbuk National University, Iksan campus)
• John Hwa Lee(College of Veterinary Medicine, Jeonbuk National University, Iksan campus) Corresponding Author