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pp.483-492
This study provides data to explore functional medicinal food materials that can prevent adult diseases, and verified antioxidant and anti-inflammatory of each solvent fraction of the methanol extract of Salvia plebeia R. Br. in Korea. In the analysis of total phenol content, DPPH radical scavenging ability, and FRAP reduction ability as indicators of antioxidant activity, the methanol fraction and ethyl acetate fraction of the Salvia plebeia R. Br. group showed high antioxidant activity. Ethyl acetate fraction of Salvia plebeia R. Br. methanol extract also showed excellent antioxidative activity as compared with BHT. In the mouse macrophage line Raw 264.7 cells, the NO production ability by LPS treatment was significantly increased in the LPS treatment group compared to the untreated group. In inflammatory reactions induced by LPS treatment in Raw 264.7 cells, inflammatory cytokines (TNF-α, PGE2, IL-1β) and NO production were decreased in the EtOAc fraction and MeOH fraction of the methanol extract of Salvia plebeia R. Br. compared to the case of LPS treatment alone. The anti-inflammatory effect was proved by significantly inhibiting the production of inflammatory cytokines. The present results suggest that Salvia plebeia R. Br. supplementation is beneficial for the suppression of antioxidant and anti-inflammation.
서 론
재료 및 방법
1. 재료 및 추출물
2. 총 페놀 함량
3. DPPH 라디칼 소거활성
4. FRAP(Ferric reducing antioxidant power) 환원능
5. 마우스 대식세포 RAW 264.7 세포주 배양
6. 생존율 분석
7. Nitric oxide(NO) 농도 측정
8. Prostaglandin E2 (PGE2) 생성 저해 활성능 측정
9. Enzyme-linked Immunosorbent Assay (ELISA)
10. 통계처리
결과 및 고찰
1. 총 페놀 함량 및 DPPH radical 소거능 분석
2. FRAP 환원능력
3. Raw 264.7 세포에서 분획별 배암차즈기 추출물에 의한 NO 생성능
4. Raw 264.7 세포에서 pro-inflammatory cytokine의 함량 분석
5. 항산화효과 인자와 항염증효과 인자와의 상관관계 분석
요약 및 결론
References
