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Effects of canine mesenchymal stem cells-derived exosomes in a mouse model of canine mammary tumor KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/404640
pp.147-155
Canine mammary tumors account for ~30% of all tumors in the female dogs and approximately 50% of the tumors are malignant. Exosomes have been the focus of great interest, as they appear to be involved in numerous important cellular processes. In this study, we examined the anti-tumor effects of canine mesenchymal stem cells-derived exosomes (MSC-exosomes) in an experimental murine mammary tumor model using canine mammary carcinoma cells, REM134. The MSC-exosomes were injected tumor site and tail vein of REM134 xenografted mice. We found that tumor size of the MSC-exosomes-treated group decreased compared to those of the only tumor group in REM134-driven tumorigenic mouse model. In addition, the MSC-exosomes-treated tumor group showed meaningfully reduced expression levels of the MMP-3, IL-1β, IL-6, and TNF-α compared to those in the tumor group. Specifically, we confirmed that the expression level of the CD133, potent cancer stem cell (CSC) markers, decreased in the MSC-exosomes-treated tumor group compared to the tumor group. This study suggests that the MSC-exosomes exhibited anti-tumor effects through downregulating CSC-related markers in the canine mammary tumor murine model. Further study is needed in the future, and we are conducting research on the detailed anti-tumor mechanism of the MSC-exosomes.
서 론
재료 및 방법
개 지방조직 유래 성체줄기세포 및 개 유선종양 세포주 배양
개 성체줄기세포의 세포 표면 특이 단백질 발현 및 중배엽 분화 유도
정량적 중합효소연쇄반응(quantitative real-time reversetranscriptase-polymerase chain reaction, qRT-PCR)
개 성체줄기세포 유래 엑소좀 수집 및 특이마커 분석
면역부전 마우스를 이용한 유선종양 형성 및 모니터링
결 과
개 지방조직 유래 성체줄기세포 배양 및 특성 분석
개 성체줄기세포 유래 엑소좀 수집 및 특성 분석
개 성체줄기세포 유래 엑소좀의 항종양 효과
고 찰
REFERENCES
