We investigated the cytotoxic potential of three different commercially available absorbent feminine hygiene products and one transdermal patch using direct contact and extract exposure methods. Two different cell lines were used – mouse fibroblast L929 and normal human skin fibroblast CCD-986sk cell lines. The test samples were extracted using three different methods in accordance to International Organization for Standardization (ISO). Viability of cells was analyzed using MTT assay and morphology of the cells were also observed using phase contrast microscopy. Overall, the direct contact method using L929 cells showed that all the test samples had no toxic effect when exposed to extracts for 1 h. For the exposure method, no toxic effect was observed in both L929 and CCD986sk cells incubated with all the test samples regardless of the extraction methods used.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Test sample extract preparation
    Cell culture
    Direct contact method
    Extract exposure method
    Statistical analysis
RESULTS AND DISCUSSION
REFERENCES

• Alisha Wehdnesday Bernardo Reyes(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Tran Xuan Ngoc Huy(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Son Hai Vu(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Trang Thi Nguyen(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Wongi Min(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Hu Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author.