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Intra-vitamlarval migration of Anisakis pegreffii in live spotty-bellied greenling, Hexagrammos otakii, at different water temperatures KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

The present study investigated the intra-vitam larval migration of Anisakis pegreffii, which is naturally infected, from the intestine to the viscera and the musculature of live spotty-bellied greenling, Hexagrammos otakii, under the different seawater temperatures. The results showed that the increase in the ratio of larval migration from the intestine to the viscera correlated with the increase in the water temperature in the aquarium. The intra-vitam larval migration ratios from the intestine to the viscera were 60% (63/105) at 15°C, 1.64% (3/183) at 12°C, 1.16% (2/172) at 9°C and 1.42% (2/141) in the control group (7.5°C). The free larvae showed no, slight and strong movement at 9°C, 12°C and 15°C, respectively, in saline water, and the larvae were present in a coiled state at 9°C and 12°C. The results suggest that temperature plays an important role in the intra-vitam larval migration of A. pegreffii. In addition, the free larval behavior of A. pegreffii in vitro increased with increase in temperature. Therefore, to prevent human anisakiasis caused by the consumption of raw fish, it is important that the temperature of the aquarium must be maintained below 12°C, which is the critical temperature zone.

목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
    Capture and harvesting of the larvae and organs of the fish
    Microscopic observation of the larvae and the fishorgans
    Genomic DNA extraction, PCR, and sequencinganalysis
RESULTS
DISCUSSION
REFERENCES
저자
  • Seo In Lee(Research Institute of Veterinary Medicine and College of Veterinary Medicine, Chungnam National University)
  • Eui Ju Hong(Research Institute of Veterinary Medicine and College of Veterinary Medicine, Chungnam National University)
  • Hyeon Cheol Kim(College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • Si Yun Ryu(Research Institute of Veterinary Medicine and College of Veterinary Medicine, Chungnam National University)
  • Bae Keun Park(Research Institute of Veterinary Medicine and College of Veterinary Medicine, Chungnam National University) Corresponding Author.